An analytical study of histological hazards estimation and comparison between cholecystitis and cholelithiasis by routine, mucin and lipid histochemistry procedures

The current study emphasizes on routine, mucin and lipid histochemistry procedures to estimate and compare histological hazards in diseased human stone filled and bacterial infections induced hypertrophied gall bladder. 100 normal & 50 (25+25) diseased human gallbladders having cholelithiasis and cholecystitis were chosen for conduction of this study. After proper processing, from each sample 3 slides were stained by H&E stain, Combined PAS-AB stain & Sudan Black B stain respectively for routine, mucin & lipid histochemistry study. In routine histochemistry predominant histological alterations were estimated and found maximum epithelial discontinuity was 62.5% in cholelithiasis where as 59.09% in cholecystitis. In mucin histochemistry combine PAS-AB stain the supra-nuclear and infra-nuclear part of the epithelium showed intense brownish blue color suggestive of predominant Sulfomucin and sailomucin effect (91 .91% cases in cholelithiasis, 87.5% cases in cholecystitis). The result of lipid histochemistry indicated accumulation of phospholipids in epithelial cells predominantly in supranuclear region in cholelithiasis. (99.09%) Scattered lipids were found in other cells and tissues in cholecystitis gall bladder. (76.76%) The basic principle in gall stone formation is accumulation of lipids and hyper secretion of acidic mucin. Sulfomucin enzyme have major role in gall stone generation. Preliminary to it is the stage when cholecystitis occurs and it shows sailomucin effect. Blockage of mucin release can prevent gall stone formation in high risk patients or during high risk period. The compounds which can regulate sailylation and sulfation might help to inhibit gall stone formation and metastasis. This needs further study and entirely new therapeutic approach. Correspondence to: Jyoti Prakash Pani, Raigad Hospital & Research Center/Sir Nandkumar Tasgaonkar Institute of Medical Science & Research Center, Raigad, Karjat, NaviMumbai, India, Tel: 8433668356; E-mail: [email protected]